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HelpTest Code UWAFB / UWAFB-MC AFB Broad Range PCR
Additional Codes
| Software | Test Code |
|---|---|
| Label Text | UWAFB |
| EPIC | LAB20465 |
| Michigan Co-Tenancy Laboratory | UWAFB |
Performing Laboratory
Michigan Co-Tenancy Laboratory at Warde Medical Laboratory (MCL)
Useful For
Direct detection of microbial DNA.
DNA is isolated from specimen and amplified by conventional PCR using a battery of broad-range primers dependent on the test type requested. Amplified products are sequenced and the organism(s) identified on the basis of sequence data.
Method Name
Polymerase Chain Reaction (PCR)
Reference Values
An interpretive report will be issued
Days and Times Test Performed
Monday through Friday
Report Available
11-14 Days
Specimen Type
~ Body Fluid
~ Bone Marrow
~ Parafin Embedded Tissue
Preferred Container
Paraffin Embedded Tissue Block
Sterile IATA-Approved Screw-Capped Plastic Container
Sterile IATA-Approved Screw-Capped Plastic Container
Preferred Volume
Body Fluid: 1 mL (Sterile IATA-Approved Screw-Capped Plastic Container)
Bone Marrow: 1 mL (Lavender Top Tube (EDTA))
Bone Marrow: 1 mL (Sterile IATA-Approved Screw-Capped Plastic Container)
Minimum Volume
Body Fluid: 0.5 mL (Sterile IATA-Approved Screw-Capped Plastic Container)
Bone Marrow: 0.5 mL (Lavender Top Tube (EDTA))
Bone Marrow: 0.5 mL (Sterile IATA-Approved Screw-Capped Plastic Container)
Parafin Embedded Tissue: 1 cubic cm (Paraffin Embedded Tissue Block)
Specimen Collection and Handling
Fresh tissues and fluids are preferred to fixed material. Tissue or fluid placed in a sterile, DNA-free container is optimal for testing. Acceptable specimen sources/types are: CSF, Non-Blood Body Fluids (Including Bone Marrow), Respiratory, Genitourinary, Fresh Tissue, Paraffin Embedded Tissue Block, E-Swabs acceptable but not preferred.
Paraffin Blocks should be shipped ambient, all other specimens should be shipped to MCL-Warde Laboratory frozen.
Specimen Stability Information
Specimen Type: Body Fluid
Refrigerated: 7 Days
Specimen Type: Bone Marrow
Refrigerated: 7 Days
Add On Capable
Yes; See Stability Info for Requirements.
CPT Code Information
|
CPT Code |
CPT Description |
CPT Disclaimer |
|---|---|---|
| 87551 | Mycobacteria species, amplified probe technique | |
| 87556 | Mycobacterium tuberculosis, complex, molecular detection, PCR |
Keywords
ACID-FAST BACILLI (AFB)
MYCOBACTERIUM TUBERCULOSIS
Non-tuberculous Mycobacteria
Mycobacterium tuberculosis complex
Mycobacterium avium complex
Mycobacterium avium complex
Rejection Information
Citrated or heparinized solutions.
Direct blood, serum, plasma and stool specimens.
Tissues floating in excess formalin.
Acceptable Alternative Container(s)
Lavender Top Tube (EDTA)