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Test Code UWAFB / UWAFB-MC AFB Broad Range PCR

Additional Codes

Software Test Code
SoftID UWAFB
EPIC LAB20465
Michigan Co-Tenancy Laboratory UWAFB

Performing Laboratory

Michigan Co-Tenancy Laboratory at Warde Medical Laboratory (MCL)

Useful For

Direct detection of microbial DNA.

DNA is isolated from specimen and amplified by conventional PCR using a battery of broad-range primers dependent on the test type requested. Amplified products are sequenced and the organism(s) identified on the basis of sequence data.

Method Name

Polymerase Chain Reaction (PCR)

Reference Values

An interpretive report will be issued

Days and Times Test Performed

Monday through Friday

Report Available

11-14 Days

Specimen Type

~ Body Fluid

~ Bone Marrow

~ Parafin Embedded Tissue

Preferred Container

Paraffin Embedded Tissue Block

Sterile IATA-Approved Screw-Capped Plastic Container

Sterile IATA-Approved Screw-Capped Plastic Container

Preferred Volume

Body Fluid: 1 mL (Sterile IATA-Approved Screw-Capped Plastic Container)

Bone Marrow: 1 mL (Lavender Top Tube (EDTA))

Bone Marrow: 1 mL (Sterile IATA-Approved Screw-Capped Plastic Container)

Minimum Volume

Body Fluid: 0.5 mL (Sterile IATA-Approved Screw-Capped Plastic Container)

Bone Marrow: 0.5 mL (Lavender Top Tube (EDTA))

Bone Marrow: 0.5 mL (Sterile IATA-Approved Screw-Capped Plastic Container)

Parafin Embedded Tissue: 1 cubic cm (Paraffin Embedded Tissue Block)

Specimen Collection and Handling

Fresh tissues and fluids are preferred to fixed material. Tissue or fluid placed in a sterile, DNA-free container is optimal for testing. Acceptable specimen sources/types are: CSF, Non-Blood Body Fluids (Including Bone Marrow), Respiratory, Genitourinary, Fresh Tissue, Paraffin Embedded Tissue Block, E-Swabs acceptable but not preferred.

Paraffin Blocks should be shipped ambient, all other specimens should be shipped to MCL-Warde Laboratory frozen.

Rejection Information

Citrated or heparinized solutions.

Direct blood, serum, plasma and stool specimens.

Tissues floating in excess formalin.

Specimen Stability Information

Specimen Type: Body Fluid

Refrigerated: 7 Days

Specimen Type: Bone Marrow

Refrigerated: 7 Days

Acceptable Alternative Container(s)

Lavender Top Tube (EDTA)

Add On Capable

Yes; See Stability Info for Requirements.

CPT Code Information

CPT Code

CPT Description

CPT Disclaimer

87551 Mycobacteria species, amplified probe technique  
87556 Mycobacterium tuberculosis, complex, molecular detection, PCR  

Keywords

ACID-FAST BACILLI (AFB)

MYCOBACTERIUM TUBERCULOSIS

Non-tuberculous Mycobacteria

Mycobacterium tuberculosis complex

Mycobacterium avium complex

Mycobacterium avium complex