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Test Code TCT THROMBIN CLOTTING TIME, PLASMA

Additional Codes

Software Test Code
Label Text                         TCT
EPIC LAB324                      

Performing Laboratory

NorDx Laboratories

Useful For

Detecting fibrinogen deficiency/dysfunction in conjunction with fibrinogen assay

This assay is affected by Heparin.

If TCT result is >30.0 seconds a reptilase time will automatically reflex and be performed at an additional charge.

Method Name

Clot-Based Assay

Reference Values

11-15 seconds

Days and Times Test Performed

Monday through Sunday

Report Available

1 Day

Available Stat

Specimen Type

Plasma

Preferred Container

Light Blue Top Tube (Sodium Citrate) – Tube must be full at time of collection.

Specimen Collection and Handling

Collect specimen as follows:

1. Draw blood in light blue-top (3.2% sodium citrate) tube(s). Tube(s) must be full upon collection.

2. Separate plasma from cells as soon as possible within 4 hours of collection in a centrifuge at 2,500 rpm for 15 minutes or 3,000 rpm for 10 minutes.

3. Using a plastic transfer pipette (do not use a glass pipette) decant the plasma to a screw-capped, plastic (polypropylene) vial. Glass vials will not be accepted.

4. Spin the plasma again at 2,500 rpm for 15 minutes or 3,000 rpm for 10 minutes. Note: Double spinning the specimen ensures platelet poor plasma (<10,000 uL)

5. Divide the plasma into as many 0.5-1.0 mL aliquots as possible and cap the vial(s) tightly.

6. Place tubes upright and flash freeze specimen(s) immediately at ≤ – 20° C or below.

7. Send specimen(s) FROZEN on dry ice.

Click here to view the Procedure for Collecting a Specimen from an A-line

Specimen Stability Information

Specimen Type: Plasma

Room Temp: 4 Hours

Note: Whole blood that remains capped and unspun is stable for up to 4 hours when stored at room temperature (18 – 24°C). Frozen plasma is stable 2 weeks @ -20°C and 12 months @ -70°C. Blue top tubes for coag assays must not be refrigerated or placed on ice.

Add On Capable

Yes

Advance Beneficiary Notice Requirements

No ABN Required

CPT Code Information

CPT Code

CPT Description

CPT Disclaimer

85635 Reptilase Test if Indicated
85670 Thrombin Time, Plasma  

Performing Laboratory Location

NorDx Laboratories

LOINC Code Information

3243-3

Rejection Information

Underfilled (<90 %), clotted, high hematocrit (>55 %), or grossly hemolyzed