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Test Code PNHFL PAROXYSMAL NOCTURNAL HEMOGLOBINURIA (PNH) FLOW CYTOMETRY

Additional Codes

Software Test Code
Label Text                                            PNHFL                                      
EPIC LAB10120

Performing Laboratory

NorDx Laboratories

Useful For

This high sensitivity flow cytometric assay is used in the diagnosis and follow-up of patients with paroxysmal nocturnal hemoglobinuria (PNH).  

 

PNH is an acquired hematopoietic stem cell disorder characterized by chronic hemolysis, venous thrombosis, and bone marrow failure.  Includes flow cytometric analysis of RBCs and WBCs using gating antibodies CD45, CD15, CD64, CD235a; GPI-linked antibodies CD59, CD14, CD24; as well as Fluorescent Aerolysin (FLAER).

Method Name

Flow Cytometry

 

This assay is a lab developed test established by the NorDx Flow Cytometry Laboratory. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity clinical laboratory testing. This test is used for clinical purposes. It should not be regarded as investigational or for research.

Reference Values

An interpretive report will be issued

Days and Times Test Performed

Monday through Friday

Report Available

3 Days

Specimen Type

Whole Blood

Preferred Container

Lavender Top Tube (EDTA)

Preferred Volume

Whole Blood: 4 mL (Lavender Top Tube (EDTA))

Minimum Volume

Collecting minimum volumes can result in a need for sample recollection, and/or a delay in results. Minimum volumes are subjective and cannot account for all aspects of specimen and testing needs. Refer to the Preferred Volume section for optimal volumes for laboratory specimens.

 

Whole Blood: 2 mL (Lavender Top Tube (EDTA))

Specimen Collection and Handling

1. Send whole blood specimen at ambient or refrigerated temperature.

2. Specimen must be received within 24 - 48 hours of collection.

3. Specimen must be received at the NorDx Scarborough facility by midnight Friday to ensure processing within 48 hours of collection.

Specimen Stability Information

Specimen Type: Whole Blood

Room Temp: 48 Hours

Refrigerated:  48 Hours

Add On Capable

Not Permitted

Advance Beneficiary Notice Requirements

No ABN Required

CPT Code Information

CPT Code

CPT Description

CPT Disclaimer
88184 Flow Cytometry, Cell Surface, Cytoplasmic, or Nuclear Marker, Technical Component Only, First Marker  
88185 x5 Flow Cytometry, Cell Surface, Cytoplasmic, or Nuclear Marker, Technical Component Only, Each Additional Marker (List Separately in Addition to Code for First Marker)  
88187 Flow Cytometery, Interpretation; 2 - 8 markers  

Keywords

PNH (PAROXYSMAL NOCTURNAL HEMOGLOBINURIA)

Performing Laboratory Location

NorDx Laboratories

Clinical Significance

Testing is indicated for: 

  • individuals with PNH symptoms (intra-vascular hemolysis or thrombosis)
  • individuals who lack PNH symptoms but have bone marrow failure diseases such as aplastic anemia, refractory anemia, or MDS
  • individuals being treated for PNH

 

This test is usually ordered in conjunction with a physician interpretation. The physician professional component will be billed separately by the consulting physician.

Rejection Information

Bone marrow specimens are not acceptable

Clotted tubes