Test Code JAK2V JAK2 V617F MUTATION DETECTION

Important Note

This test may require pre-authorization or have limited coverage. Please check with your appropriate insurance carrier to determine any specific requirements.

Additional Codes

Software	Test Code
Label Text	JAK2V
EPIC	LAB10272

Performing Laboratory

NorDx Laboratories

Useful For

Differentiating P.vera and other chronic myeloproliferative disorders from reactive processes

Indications for testing are reviewed in: Steensma DP. JAK2 V617F in Myeloid Disorders: Molecular Diagnostic Techniques and Their Clinical Utility. J Mol Diagn. 2006;8:397-411.

Method Name

Real-Time Polymerase Chain Reaction (rt-PCR) Qualitative

This assay is a lab developed test established by the NorDx Molecular Pathology Laboratory. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity clinical laboratory testing. This test is used for clinical purposes. It should not be regarded as investigational or for research.

Reference Values

Jak2 mutation is detected or not detected

Days and Times Test Performed

Tuesday

Report Available

1 week

Profile Information

Individual Test Description
INTERPRETATION, JAK2

Specimen Type

Submit one of the following:

~ Bone Marrow

~ Whole Blood

Preferred Container

Lavender Top Tube (EDTA) – Bone Marrow

Lavender Top Tube (EDTA) – Whole Blood

Preferred Volume

Bone Marrow: 4 mL (Lavender Top Tube (EDTA))

Whole Blood: 4 mL (Lavender Top Tube (EDTA))

Minimum Volume

Bone Marrow: 0.5 mL (Lavender Top Tube (EDTA))

Whole Blood: 0.4 mL (Lavender Top Tube (EDTA))

Specimen Collection and Handling

1. Invert lavender top tube several times to mix.

2. Send at ambient temperature but do not freeze.

Specimen Stability Information

Specimen Type: Bone Marrow

Refrigerated: 1 Week

Specimen Type: Whole Blood

Refrigerated: 1 Week

Add On Capable

Yes

Advance Beneficiary Notice Requirements

This test, when ordered on Medicare patients, is subject to the National Coverage Determination (NCD) policies. Please verify that the diagnosis code (ICD code) you have chosen demonstrates medical necessity for the test as documented in the physician’s patient record. The CMS web site is available to assist you with this verification. A properly executed Advance Beneficiary Notice (ABN) must be submitted with the specimen if medical necessity is not demonstrated by the ICD code chosen.

CPT Code Information

CPT Code	CPT Description	CPT Disclaimer
81270	JAK2 (Janus kinase 2)Gene Analysis, p.Val617Phe (V617F) Variant

Keywords

JANUS KINASE 2 GENE

TYROSINE KINASE MUTATION

Performing Laboratory Location