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HelpTest Code FVIII FACTOR VIII (8) ASSAY
Additional Codes
| Software | Test Code |
|---|---|
| Label Text | FVIII |
| EPIC | LAB306 |
Performing Laboratory
NorDx Laboratories
Useful For
Measuring factor VIII (8) activity.
Method Name
Clot-Based Assay
Reference Values
FACTOR VIII
Adults: 50-200%
Days and Times Test Performed
Monday through Friday, 8:00 am-3:00 pm
Report Available
Approximately 48 hours for STAT collections
Approximately 1 week for routine collections
Specimen Type
Plasma
Preferred Container
Light Blue Top Tube (Sodium Citrate)
Specimen Collection and Handling
1. Draw blood in light blue-top (3.2% sodium citrate) tube(s). Tube(s) must be full upon collection.
2. Separate plasma from cells as soon as possible within 4 hours of collection in a centrifuge at 2,500 rpm for 15 minutes or 3,000 rpm for 10 minutes.
3. Using a plastic transfer pipette (do not use a glass pipette) decant the plasma to a screw-capped, plastic (polypropylene) vial. Glass vials will not be accepted.
4. Spin the plasma again at 2,500 rpm for 15 minutes or 3,000 rpm for 10 minutes. Note: Double spinning the specimen ensures platelet poor plasma (<10,000 uL)
5. Divide the plasma into as many 0.5-1.0 mL aliquots as possible and cap the vial(s) tightly.
6. Place tubes upright and flash freeze specimen(s) immediately at ≤ – 20° C or below.
7. Send specimen(s) FROZEN on dry ice.
Click here to view the Procedure for Collecting a Specimen from an A-line
Specimen Stability Information
Specimen Type: Plasma
Room Temp: 4 Hours
Note: Whole blood that remains capped and unspun is stable for up to 4 hours when stored at room temperature (18 – 24°C). Frozen plasma is stable 2 weeks @ -20°C and 12 months @ -70°C. Blue top tubes for factor assays must not be refrigerated or placed on ice.
Add On Capable
Yes – if testing is added before the specimen is past 4 hour stability limit at room temperature. Add on testing also possible if plasma is still available frozen @ -70°C. Please contact the Bramhall Special Coagulation Laboratory to verify that frozen plasma is still available to perform the request.
Advance Beneficiary Notice Requirements
No ABN Required
CPT Code Information
|
CPT Code |
CPT Description |
CPT Disclaimer |
|---|---|---|
| 85240 | Clotting, Factor VIII (AHG), 1-stage |
Keywords
FACTOR ACTIVITY
FACTOR 8
FACTOR LEVEL
COAGULATION FACTOR ASSAY
Clinical Significance
Coagulation testing is highly complex, often requiring the performance of multiple assays and correlation with clinical information.
Rejection Information
Specimens will be rejected if under filled (<90%), clotted, high hematocrit (>55%), or grossly hemolyzed.