Test Code FLHCL FLOW CYTOMETRY HAIRY CELL LEUKEMIA PANEL

Additional Codes

Software	Test Code
Label Text	FLHCL
EPIC	LAB10875

Performing Laboratory

NorDx Laboratories

Useful For

Diagnosis of Hairy Cell Leukemia

Method Name

Flow Cytometry

This assay is a lab developed test established by the NorDx Flow Cytometry Laboratory. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity clinical laboratory testing. This test is used for clinical purposes. It should not be regarded as investigational or for research.

Reference Values

An interpretive report will be issued

Days and Times Test Performed

Monday through Friday

Specimens drawn on Friday MUST ARRIVE in Scarborough lab before midnight Friday to ensure processing within 48 hours of collection.

Report Available

3 Days

Specimen Type

Submit one of the following:

~ Whole Blood – Both sodium heparin and EDTA whole blood are preferred for this test. See also minimum volume section.

~ Body Fluid

~ Bone Marrow

~ Cerebrospinal Fluid (CSF)

~ Tissue – Fresh

Preferred Container

Whole Blood:

Green Top Tube (Sodium Heparin) – Whole Blood

Lavender Top Tube (EDTA) – Whole Blood

Bone Marrow Collection Tube (Special Sodium Heparin with RPMI) – Bone Marrow

Sterile Container – Body Fluid

Sterile Container – CSF

Sterile Container with RPMI Media – Tissue

Preferred Volume

Body Fluid: 50-100 mL (Sterile Container)

Bone Marrow: 3 mL (Bone Marrow Collection Tube)

Cerebrospinal Fluid (CSF): 2 - 5 mL (Sterile Container)

Tissue: 10 mm (Sterile Container with RPMI Media)

Whole Blood: 10 mL (Green Top Tube (Sodium Heparin)) AND

Whole Blood: 4 mL (Lavender Top Tube (EDTA))

Minimum Volume

Collecting minimum volumes can result in a need for sample recollection, and/or a delay in results. Minimum volumes are subjective and cannot account for all aspects of specimen and testing needs. Refer to the Preferred Volume section for optimal volumes for laboratory specimens.

Body Fluid: 25 mL (Sterile Container)

Bone Marrow: 1 mL (Bone Marrow Collection Tube)

Cerebrospinal Fluid (CSF): 1 mL (Sterile Container)

Tissue: 2 mm (Sterile Container with RPMI Media)

Whole Blood: 2 mL (Lavender Top Tube (EDTA))

Specimen Collection and Handling

BLOOD:

Both sodium heparin and EDTA whole blood are required for this test. Draw blood in green-top (sodium heparin) tube(s), and lavender-top tube(s). Send 10 mL of sodium heparin whole blood and 4 mL of EDTA whole blood at ambient temperature only. CLOTTED SPECIMEN IS UNACCEPTABLE.

BONE MARROW:

Submit 1 - 3 mL if bone marrow in a special bone marrow collection tube, available by calling the NorDx Flow Cytometry Lab (207-396-7912). Forward promptly at ambient temperature only.

TISSUE:

The amount of tissue needed is dependent on the cellularity of the specimen. Generally, a 2-10 mm section of fresh tissue is adequate. Place fresh tissue in a container with RPMI media (supplied by NorDx Flow Cytometry lab, 207-396-7912). TISSUE RECEIVED IN A FIXATIVE IS NOT ACCEPTABLE. Label container with patient’s name (first and last), date and time of collection, and type of specimen. Forward promptly at ambient temperature only.

FLUID:

Pleural, peritoneal, pericardial or BAL fluid collected in a sterile container. The amount of fluid required is dependent on cell count, but as a general rule 50-100 mL of fluid is required. Forward promptly at ambient temperature only.

CSF:

CSF collected in a sterile container. Preferred sample volume is 2-5 mL. Forward promptly at ambient temperature only.

Date of collection is required on the request form for processing.