ENTEROVIRUS DETECTION, REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION (RT-PCR)

Additional Codes

Software	Test Code
Label Text	EVPCR
EPIC	LAB11290

Performing Laboratory

NorDx Laboratories

Useful For

Diagnosis of Enterovirus infection

This assay detects human Enteroviruses, including the EV-D68, Coxsackie viruses, Polioviruses and the Echoviruses. It does not differentiate among the EV serotypes.

Method Name

Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Quantitative

This assay is a lab developed test established by the NorDx Molecular Pathology Laboratory. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity clinical laboratory testing. This test is used for clinical purposes. It should not be regarded as investigational or for research.

Reference Values

Not detected

Days and Times Test Performed

Monday through Saturday

-CSF

Monday, Wednesday and Friday

-Other specimen

Report Available

1 Day

Available Stat

Profile Information

Individual Test Description
ENTEROVIRUS PCR, RESULT
ENTEROVIRUS PCR, SPECIMEN SOURCE

Specimen Type

Submit one of the following:

~ Cerebrospinal Fluid (CSF)

~ Plasma

~ Swab from Affected Area

Preferred Container

Lavender Top Tube (EDTA) – Plasma

Screw-Capped Sterile Vial – CSF

Note: For Add-on contact Molecular (HSVQL specimen in Red Top Tube in Molecular can be used for EVPCR add-on)

UTM Collection Kit – NASOPHARYNGEAL, DERMAL, RECTAL, or other sites clinically indicated.

Preferred Volume

Cerebrospinal Fluid (CSF): 1 mL (Screw-Capped Sterile Vial)

Plasma: 1 mL (Lavender Top Tube (EDTA))

Minimum Volume

Cerebrospinal Fluid (CSF): 0.5 mL (Screw-Capped Sterile Vial)

Plasma: 0.5 mL (Lavender Top Tube (EDTA))

Specimen Collection and Handling

PLASMA:

1. Spin specimen, separate from clot and send refrigerated.

2. Specimen source is required on request form for processing.

CSF:

1. Specimens grossly contaminated with blood may inhibit the PCR and produce false negative results.

2. Send CSF refrigerated.

SWAB:

NASOPHARYNGEAL, DERMAL, RECTAL, or other sites clinically indicated.

1. Collect specimen from the affected site using the flocked swab provided with red top UTM collection kit.

2. Label with the specimen source and send refrigerated.

3. Dry swab or swab containing gel is not acceptable for PCR testing.

Specimen Stability Information

Specimen Type: Cerebrospinal Fluid (CSF)

Note: CSF ADD-ON REQUESTS: HSVQL SPECIMEN CAN BE USED FOR ADD-ON, CONTACT TESTING SECTION

Specimen Type: Swab from Affected Area

Frozen: 1 Month

Refrigerated: 7 Days

Add On Capable

Yes

Advance Beneficiary Notice Requirements

No ABN Required

CPT Code Information

CPT Code	CPT Description	CPT Disclaimer
87498	Infectious Agent Detection by Nucleic Acid (DNA or RNA), Enterovirus, Amplified Probe Technique, Includes Reverse Transcription when Performed

Performing Laboratory Location