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Performing Laboratory

Michigan Co-Tenancy Laboratory at Warde Medical Laboratory (MCL)

Useful For

Diagnosing dermatitis herpetiformis and celiac disease and for monitoring adherence to gluten-free diet in patients with dermatitis herpetiformis and celiac disease

Clinical Significance

Because of the high specificity of endomysial antibodies for celiac disease, the test may obviate the need for multiple small bowel biopsies to verify the diagnosis. This may be particularly advantageous in the pediatric population, including the evaluation of children with failure to thrive.

Method Name

Indirect Immunofluorescence Antibody (IFA)

Reference Values

<1:10 Negative

Days and Times Test Performed

Monday through Friday

Analytic Time

3 Days

Specimen Type


Preferred Container

Serum Separator Tube (SST)

Preferred Volume

Serum: 1 mL (Serum Separator Tube (SST))

Minimum Volume

Serum: 0.5 mL (Serum Separator Tube (SST))

Specimen Collection and Handling

Spin specimen, separate from clot and send refrigerated in a screw-capped, plastic vial.

Specimen Stability Information

Specimen Type: Serum

Frozen: 14 Days

Refrigerated: 2 Days

Add On Capable

Contact Reference Lab – If add-on can never be done, report the test to the Administrative Analyst

Advance Beneficiary Notice Requirements

No ABN Required

CPT Code Information

CPT Code

CPT Description

CPT Disclaimer

86255 Flourescent Noninfectious Agent Antibody, Each Antibody
86256 Fluorescent Noninfectious Agent Antibody, Titer, Each Antibody

Acceptable Alternative Container(s)

Red Top Tube

LOINC Code Information


Performing Laboratory Location

Michigan Co-Tenancy Laboratory at Warde Medical Laboratory (MCL)