Test Code APPAN ANTIPHOSPHOLIPID AB PANEL
Additional Codes
Software | Test Code |
---|---|
Label Text | APPAN |
EPIC | LAB20117 |
Label Text | CAPPAN - Chemistry Portion of Panel |
Performing Laboratory
NorDx Laboratories
Useful For
The detection of lupus anticoagulant in a patient with a prolonged PTT
Panel components:
Interpretation
INR,plasma
PTT
Silica Screen
Silica Confirmation
DRVVT Screen
DRVVT Confirmation
Hematocrit (will be performed but not charged)
CAPPAN labeled specimens:
Cardiolipin Antibodies, IgG and IgM
Beta-2-Glycoprotein 1 Antibodies, IgG, and IgM
If lupus anticoagulant screen is abnormal, the following tests will be performed at an additional charge:
DRVVT Mixing Study
Silica Mixing Study
PTT using reagent more sensitive to factor deficiencies (Dade Actin FS)
Thrombin Time
Method Name
Automated Clot-Based Assay
Enzyme Linked Immunosorbent Assay (ELISA)
Reference Values
An interpretive report will be issued.
Days and Times Test Performed
1 day weekly
(Usually Friday)
Report Available
14 Days
Specimen Type
Submit all of the following:
~ Plasma
~ Serum
~ Whole Blood
Preferred Container
Lavender Top Tube (EDTA)
Light Blue Top Tube (Sodium Citrate) – Collect 3 tubes – each must be full at time of collection.
Serum Separator Tube (SST)
Specimen Collection and Handling
BLUE TOP TUBES:
Collect specimen as follows:
1. Draw blood in 3 light blue-top (3.2% sodium citrate) tube(s). Tube(s) must be full upon collection.
2. Separate plasma from cells within 4 hours of collection in a centrifuge at 2,500 rpm for 15 minutes or 3,000 rpm for 10 minutes.
3. Using a plastic transfer pipette (do not use a glass pipette) decant the plasma to a screw-capped, plastic (polypropylene) vial. Glass vials will not be accepted.
4. Spin the plasma again at 2,500 rpm for 15 minutes or 3,000 rpm for 10 minutes.
Note: Double spinning the specimen ensures platelet poor plasma (<10,000 uL)
5. Divide the plasma into as many 0.5-1.0 mL aliquots as possible and cap the vial(s) tightly.
6. Place tubes upright and freeze specimen(s) immediately at ≤ – 20º C.
7. Send specimen(s) FROZEN on dry ice.
Patients with a Hematocrit >55% will require recollection in a citrate-adjusted tube.
Coagulation Double Spin Process
Click here to view the Procedure for Collecting a Specimen from an A-line
LAVENDER TOP TUBE:
1. Mix well, and send a minimum of 1mL of EDTA whole blood refrigerated.
2. To be used for a hematocrit that is run at no charge and will be non-reportable.
3. To be tested to rule out abnormal results due to a high hematocrit (> 55%).
4. If a high hematocrit is detected the client will be contacted to determine the need for a recollect in properly adjusted blue top tubes. A coagulation pathologist will assess this need with the client.
SERUM SEPARATOR TUBE:
1. Spin specimen, separate from clot within 2 hours and send refrigerated in a screw-capped, plastic vial.
2. If specimen will not arrive within 2 days of draw, send specimen FROZEN in a plastic vial on dry ice.
Rejection Information
Underfilled (<90 %), clotted, or grossly hemolyzed samples
Specimen Stability Information
Specimen Type: Plasma
Room Temp: 4 Hours
Note: Whole blood that remains capped and unspun is stable for up to 4 hours when stored at room temperature (18 – 24°C). Frozen plasma is stable 2 weeks @ -20°C and 12 months @ -70°C. Blue top tubes for coag assays must not be refrigerated or placed on ice.
Specimen Type: Serum
Refrigerated: 48 Hours
Must be spun/separated within: 2 Hours
Specimen Type: Whole Blood
Refrigerated: 24 Hours
Add On Capable
Not Permitted
Advance Beneficiary Notice Requirements
This test, when ordered on Medicare patients, is subject to the National Coverage Determination (NCD) policies. Please verify that the diagnosis code (ICD code) you have chosen demonstrates medical necessity for the test as documented in the physician’s patient record. The CMS web site is available to assist you with this verification. A properly executed Advance Beneficiary Notice (ABN) must be submitted with the specimen if medical necessity is not demonstrated by the ICD code chosen.
CPT Code Information
CPT Code |
CPT Description |
CPT Disclaimer |
---|---|---|
84285 | Assay of Silica, Quantitative | |
84285 | Assay of Silica, Quantitative | if Indicated |
84285-59 | Assay of Silica, Quantitative | |
85610 | Prothrombin Time | |
85613 | Russell Viper Venom Time (Includes Venom); Diluted | |
85635 | Reptilase Test | |
85670 | Thrombin Time, Plasma | |
85670 | Thrombin Time, Plasma | if Indicated |
85730 | Thromboplastin Time, Partial (PTT), Plasma or Whole Blood | |
85730 | Thromboplastin Time, Partial (PTT), Plasma or Whole Blood | if Indicated |
85732 | Thromboplastin Time, Partial (PTT), Plasma Fractions, Each | |
86146 | Beta 2 Glycoprotein 1 Antibody, Each | |
86147 | Cardiolipin (Phospholipid) Antibody, Each Ig Class |
Acceptable Alternative Container(s)
Red Top Tube (can replace SST only)
Performing Laboratory Location
NorDx Laboratories