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HelpTest Code ALKP / ALKP-M Alkaline Phosphatase, Total and Isoenzymes, Serum
Additional Codes
| Software | Test Code |
|---|---|
| Label Text | ALKP |
| EPIC | LAB9413 |
| Mayo Laboratories | ALKP |
Reporting Name
Alkaline Phosphatase, Tot and Iso,SUseful For
Aid in the diagnosis and treatment of liver, bone, intestinal, and parathyroid diseases
Determining the tissue source of increased alkaline phosphatase (ALP) activity in serum
Differentiating between liver and bone sources of elevated ALP
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ALP | Alkaline Phosphatase, S | Yes | Yes |
| ALPI | Alkaline Phosphatase Isoenzymes, S | No | Yes |
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumNecessary Information
Patient's age and sex are required.
Specimen Required
Patient Preparation: Patient must fast for 8 hours.
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Within 2 hours of collection, centrifuge the specimen.
2. For red top tubes, immediately aliquot into a plastic vial.
3. For serum gel tubes, serum may sit on gel refrigerated but must be aliquoted within 7 days.
Specimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Frozen (preferred) | 14 days |
| Ambient | 7 days | |
| Refrigerated | 7 days |
Reference Values
ALKALINE PHOSPHATASE
Males
0-14 days: 83-248 U/L
15 days-<1 year: 122-469 U/L
1-<10 years: 142-335 U/L
10-<13 years: 129-417 U/L
13-<15 years: 116-468 U/L
15-<17 years: 82-331 U/L
17-<19 years: 55-149 U/L
≥19 years: 40-129 U/L
Females
0-14 days: 83-248 U/L
15 days-<1 year: 122-469 U/L
1-<10 years: 142-335 U/L
10-<13 years: 129-417 U/L
13-<15 years: 57-254 U/L
15-<17 years: 50-117 U/L
≥17 years: 35-104 U/L
ALKALINE PHOSPHATASE ISOENZYMES
Ages:
≤17 years: Reference values have not been established for patients younger than 18 years.
≥18 years:
Liver %: 30.2-74.7
Liver U/L: 15.8-71.9
Bone %: 23.8-68.3
Bone U/L: 12.0-56.7
Intestine %: ≤22.5
Intestine U/L: ≤12.6
Day(s) Performed
Tuesday through Saturday
CPT Code Information
84075
84080
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| ALKP | Alkaline Phosphatase, Tot and Iso,S | 24332-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| ALP | Alkaline Phosphatase, S | 6768-6 |
| 622367 | Alkaline Phosphatase Isoenzymes, S | 12805-8 |
| 622369 | Liver Percent | 15015-1 |
| 622368 | Liver | 1779-8 |
| 622371 | Bone Percent | 15013-6 |
| 622370 | Bone | 1777-2 |
| 622373 | Intestine Percent | 15014-4 |
| 622372 | Intestine | 1778-0 |
Report Available
3 to 5 daysReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | Reject |
Secondary ID
622157Method Name
ALP: Colorimetric
ALPI: Electrophoresis
Clinical Information
Serum alkaline phosphatase (ALP) is used in the diagnosis of hepatobiliary disease and bone disease associated with increased osteoblastic activity. ALP is present in a number of tissues including liver, bone, intestine, and placenta. The activity of ALP found in serum is a composite of isoenzymes from those sites.
A rise in liver ALP activity occurs with all forms of cholestasis, particularly with obstructive jaundice.
Bone ALP is elevated in disorders of the skeletal system that involve osteoblast hyperactivity and bone remodeling, such as Paget disease, rickets, osteomalacia, fractures, and malignant tumors.
Moderate elevation of ALP may be seen in other disorders such as Hodgkin disease, congestive heart failure, ulcerative colitis, regional enteritis, and intra-abdominal bacterial infections.
Cautions
Alkaline phosphatase (ALP) Isoenzyme:
-High concentrations of phosphate, oxalate, citrate, and cyanide will inhibit ALP activity.
-Excess glycine may inhibit ALP activity by complexing magnesium.
-Patients should be fasting. Patients may have an elevated intestinal ALP about two hours after a fatty meal.
Method Description
Total Alkaline Phosphatase:
In the presence of magnesium and zinc ions, p-nitrophenyl phosphate is cleaved by phosphatases into phosphate and p-nitrophenol. The p-nitrophenol released is directly proportional to the catalytic alkaline phosphatase (ALP) activity. It is determined by measuring the increase in absorbance.(Package insert: Alkaline Phosphatase reagent. Roche Diagnostics; V 9.0 10/2021)
Alkaline phosphatase Isoenzymes:
Alkaline phosphatase isoenzymes are separated by agarose gel electrophoresis and visualized using BCIP (5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt) substrate.(Package insert: SPIFE Touch Alkaline Phosphatase [ALP] Isoenzyme. Helena Laboratories; 01/2024)