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Test Code ACTHH CORTROSYN STIMULATION TEST (0, 1/2 HOUR)

Additional Codes

Software Test Code
Label Text ACTHH
EPIC LAB10179

Performing Laboratory

NorDx Laboratories

Useful For

The evaluation of adrenal insufficiency in suspected adrenal biosynthetic defect or adrenocorticotropic hormone (ACTH) unresponsiveness.

Test includes two serum cortisol determinations--one at 0 minutes and one at 30 minutes.

Method Name

Electrochemiluminescent Immunoassay (ECLIA)

Reference Values

Stimulated cortisol level: >18 ug/dL and/or a >7 ug/dL increase over the baseline level

Days and Times Test Performed

Monday through Friday

Report Available

1 Day

Profile Information

Individual Test Description
CORTISOL BASELINE
CORTISOL, 30 MINUTES

Specimen Type

Serum

Preferred Container

Serum Separator Tube (SST)

Preferred Volume

Serum: 1 mL (Serum Separator Tube (SST))

Minimum Volume

Serum: 0.5 mL (Serum Separator Tube (SST))

Specimen Collection and Handling

1. Draw baseline blood in a serum gel tube.

2. Immediately after drawing baseline, clinician administers 0.25 mg ACTH (Cortrosyn).

3. Draw second blood specimen 30 minutes post injection.

4. Label tubes with the collection time.

To Process:

1. Spin down and send 1.0 mL serum from each specimen (pre and post injection) refrigerated.

2. Label tubes with collection time.

Specimen Stability Information

Specimen Type: Serum

Frozen: 3 Months

Refrigerated: 4 Days

Must be spun/separated within: 2 Hours

Add On Capable

Not Permitted

Advance Beneficiary Notice Requirements

No ABN Required

CPT Code Information

CPT Code

CPT Description

CPT Disclaimer
80400 ACTH Stimulation Panel for Adrenal Insufficiency (Must include Cortisol)  

Performing Laboratory Location

NorDx Laboratories

Clinical Significance

No sample should be collected on patients receiving therapy with high biotin doses (i.e. biotin therapy for Multiple Sclerosis or oncology patients; skin, hair and nail supplements, or multivitamins containing > 5 mg/day) until at least 12 hours after the last biotin administration. If unsure, or if the clinical picture does not fit the results please contact the laboratory . We have methods to check for biotin interference as well as for any other interferences and for the accuracy of the results.

Biotin interference would falsely increase the result of this assay.

Acceptable Alternative Container(s)

Plasma Separator Tube (PST)

Red Top Tube