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Test Code LAP LEUKOCYTE ALKALINE PHOSPHATASE (LAP)

Performing Laboratory

NorDx Laboratories

Useful For

Differentiating chronic granulocytic leukemia from leukemoid reaction

Method Name

Enzymatic Activity

Reference Values

Score of 15-100 Units

Days and Times Test Performed

Monday through Sunday

Analytic Time

1 Day

Available Stat

Specimen Type

Submit one of the following:

~ Peripheral Smear

~ Whole Blood

Preferred Container

Dark Green Top Tube (Lithium Heparin)

Peripheral Blood Smear – Smears must be made within 1 hour of collection.

Preferred Volume

Peripheral Smear: 6 slide (Peripheral Blood Smear)

Whole Blood: 5 mL (Dark Green Top Tube (Lithium Heparin))

Minimum Volume

Whole Blood: 1 mL (Dark Green Top Tube (Lithium Heparin))

Specimen Collection and Handling

Submit only one of the following specimens:

BLOOD:

1. Draw blood in a green-top (Li Heparin) tube, and immediately forward 3.0 – 5.0 mL of heparinized whole blood to the Bramhall Hematology Laboratory.

2. SPECIMEN MUST ARRIVE WITHIN 1 HOUR OF DRAW.

3. If specimen will not arrive within 1 hour of draw, prepare and send peripheral blood smears.

SMEARS:

1. Submit 6 well-made peripheral blood smears.

2. Smears should be stained for enzyme activity within 8 – 12 hours of preparation.

3. Loss of activity may occur when there is a delay.

4. Send slides in plastic slide container.

Specimen Stability Information

Specimen Type: Peripheral Smear

Refrigerated: 1 Hour

Note: Smears must be made within 1 hour of sample collection.

Specimen Type: Whole Blood

Refrigerated: 1 Hour

Note: Smears must be made within 1 hour of sample collection.

Add On Capable

Not Permitted

Advance Beneficiary Notice Requirements

No ABN Required

CPT Code Information

CPT Code

CPT Description

CPT Disclaimer

85540 Leukocyte Alkaline Phosphatase with Count

LOINC Code Information

15112-6

Keywords

ALKALINE PHOSPHATASE, LEUKOCYTE

CHRONIC GRANULOCYTIC LEUKEMIA

LAP (LEUKOCYTE ALKALINE PHOSPHATASE)

Performing Laboratory Location

NorDx Laboratories